|
产品编号
R4875 |
CAS
号
9001-99-4
|
Sigma
Ribonuclease A from bovine pancreas
Type I-A, powder,
≥
60% RNase A basis (SDS-PAGE),
≥
50 Kunitz units/mg protein
别名
:
RNase A
核糖核酸
3′-
嘧啶寡核苷酸水解酶
核糖核酸酶
I
胰核糖核酸酶
价格与库存
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货号与规格
|
库存
|
|
价格
(RMB)
|
数量
|
|
|
|
R4875-50mg
|
国内现货或即将上架，预计发货
2013.07.19 -
详情
|
|
320.00
225.00
|
现货
|
|
|
|
R4875-100MG
|
国内现货或即将上架，预计发货
2013.07.19 -
详情
|
|
560.00
390.00
|
现货
|
|
|
|
AC117-400ul
|
国内现货或即将上架，预计发货
2013.07.19 -
详情
|
|
150.00
|
现货
|
|
|
产品描述
Analysis Note
通过
E1%/280
确定的蛋白质。
• RNase protection assays
• Remove unspecifically bound RNA
• Analysis of RNA sequences
• Hydrolyze RNA contained in protein samples
• Purification of DNA
Preparation Note
Salt fractionated and chromatographically purified.
Application
Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays. RNase has been used as a tool for computer-aided drug design .
Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.
Ribonuclease A is an endoribonuclease that cleaves single stranded RNA after pyrimidine nucleotides. It attacks at the 3’ phosphate end. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12
and His119
and activated by potassium and sodium salts.
性质
|
Related Categories
|
3.1.x.x Acting on esters, 3.x.x.x Hydrolases,Application Index, Biochemicals and Reagents, Cell Biology,
|
|
type
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Type I-A
|
|
assay
|
≥60% RNase A basis (SDS-PAGE)
|
|
form
|
powder
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mol wt
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mol wt ~13,700
|
|
impurities
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salt, essentially free
|
|
foreign activity
|
protease, essentially free
|
|
storage temp.
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−
20°C
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|
